pFast-Bac-HTC质粒中特生物供应

 基本信息
启动子:PC,polyhedrin复制子:pUC ori，F1 ori终止子:SV40 poly(A) signal质粒分类:广宿主系列，昆虫细胞载体质粒大小:4858bp质粒标签:N-His；N-TEV原核抗性:Amp筛选标记:Gen克隆菌株:DH5α培养条件:37℃，有氧 LB表达宿主:昆虫细胞诱导方式:无须诱导，瞬时表达5'测序引物:pFastbac-F: TATTCCGGATTATTCATACC3'测序引物:pFastBac-R: ACAAATGTGGTATGGCTGA
质粒简介      The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.

质粒图谱 

 

质粒序列

LOCUS       Exported                4858 bp ds-DNA     circular SYN 07-SEP-2016

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    Untitled 11

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4858)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Wednesday, September 7, 2016 from SnapGene Viewer 3.1.4

            http://www.miaolingbio.com

FEATURES             Location/Qualifiers

     source          1..4858

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      2..457

                     /direction=RIGHT

                     /note="f1 ori"

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        484..588

                     /gene="bla"

                     /note="AmpR promoter"

     CDS             589..1449

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      1620..2208

                     /direction=RIGHT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     mobile_element  2511..2735

                     /mobile_element_type="transposon:Tn7"

                     /note="Tn7R"

                     /note="mini-Tn7 element (right end of the Tn7 transposon)"

     CDS             complement(2802..3335)

                     /codon_start=1

                     /gene="aacC1"

                     /product="gentamycin acetyltransferase"

                     /note="GmR"

                     /note="confers resistance to gentamycin"

                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD

                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS

                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR

                     EEVMHFDIDPSTAT"

     promoter        complement(3524..3552)

                     /gene="intI1 (promoter lies within the coding sequence)"

                     /note="Pc promoter"

                     /note="class 1 integron promoter"

     promoter        3904..3995

                     /gene="polh from Autographa californica"

                     /note="polyhedrin promoter"

                     /note="promoter for the baculovirus polyhedrin gene"

     CDS             4062..4079

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /note="6xHis"

                     /translation="HHHHHH"

     CDS             4101..4121

                     /codon_start=1

                     /product="tobacco etch virus (TEV) protease recognition and

                     cleavage site"

                     /note="TEV site"

                     /translation="ENLYFQG"

     polyA_signal    4348..4482

                     /note="SV40 poly(A) signal"

                     /note="SV40 polyadenylation signal"

     mobile_element  4511..4676

                     /mobile_element_type="transposon:Tn7"

                     /note="Tn7L"

                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

ORIGIN

        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc

       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc

      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt

      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg

      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt

      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta

      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt

      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat

      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg

      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa

      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac

      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac

      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt

      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc

      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca

      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc

      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag

     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa

     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg

     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa

     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg

     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt

     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt

     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag

     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat

     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct

     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc

     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc

     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg

     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag

     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt

     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac

     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg

     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc

     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg

     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct

     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga

     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag

     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt

     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga

     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac

     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg

     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg

     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca

     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact

     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc

     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta

     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct

     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg

     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg

     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca

     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa

     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa

     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca

     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac

     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc

     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg

     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt

     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt

     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa

     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt

     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca

     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg

     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatgggg atccggaatt

     4141 caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt

     4201 ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc

     4261 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa

     4321 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa

     4381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt

     4441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact agatctgcct

     4501 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt

     4561 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa

     4621 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg

     4681 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc

     4741 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt

     4801 attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatgg