直链淀粉/支链淀粉[胶淀粉]检测试剂盒K-AMYL

Megazyme检测试剂盒优点: 试剂盒新颖，试剂稳定

For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. based on a Con A precipitation procedure. Content:100 assays per kit

Colourimetric method for the determination of Amylose and 
Amylopectin in cereals, food and feed

Principle:
(Con A)
(1) Soluble starch (amylose + amylopectin) → 
amylose + amylopectin-Con A
(soluble) (precipitate)

(α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
quinoneimine + H2O

(α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H2O → D-glucose

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:               ~ 120 min
Detection limit:                  Amylose 5-95% of total starch content
Application examples: 
Cereal starches, flours, pure starches and foods
Method recognition:          Novel method

Advantages

• Very cost effective (cost per test)
  
• All reagents stable for > 12 months after preparation
  
• only enzymatic kit available
  
• Accurate and reliable amylose / amylopectin ratio determination 
  
• Simple format
  
• Standard included