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描述
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour.• Formulated for use with liquid samples such as serum and virus preparations
• Facilitates recovery of RNA, DNA, and protein from a single liquid sample
• Offers excellent lysis capability, even with difficult biological fluids
Reliably Purify RNA from Multiple Sample Sources
TRIzol® LS Reagent is designed for processing a variety of liquid samples of up to 0.25 ml in volume. TRIzol® LS Reagent differs from the standard TRIzol® Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRIzol® Reagent, the integrity of resulting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRIzol® LS Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® LS Reagent is free of protein and DNA contamination.
Formulated to Serve Multiple Isolations
TRIzol LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
规格
| Sample Type (General): | Liquid Samples (e.g. Serum), Viral Samples, Blood |
|---|---|
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Downstream Application: | Reverse Transcriptase PCR (RT-PCR), Northern Blotting, Cloning, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays, cDNA Library Construction |
| Scale: | Scaleable |
| System: | TRIzol |
| Quantity: | 1 |
| Product Size: | 100 mL |
| Final Product: | Total RNA, micro RNA, Transcriptome RNA |
| Yield (up to): | 8 mg |
| Purification Time: | 1 hr |
| Number of Reactions: | 100 Preps |
| Isolation Technology: | Organic Extraction |
| Starting Material (Amount): | 250 µl to 1 ml liquid samples (suggested range; scalable) |
| Shipping Condition: | Room Temperature |
内容及储存
Includes TRIzol® LS Reagent. Store at 2°C to 8°C.
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