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沙门氏菌检测卡（食品安全检测）

沙门氏菌检测卡（食品安全检测）

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2, Inclusion body dissolved

Sodium lauryl sarcosinate and lauryl sarcosine in the dissolution of inclusion bodies, can replace each other? Can be replaced, adjust pH no difference in the near future it; both the same functional groups, pH is different, the former sodium salt easy to save Bale

3, the dissolution of inclusion bodies

Strong denaturants such as urea (6-8M), guanidine hydrochloride (GdnHCl 6M), is through the inter-ionic interactions, breaking the inclusion body of protein molecules within and between various chemical bonds, the peptide stretch, in general, Guanidine hydrochloride is superior to urea because guanidine hydrochloride is a more urea-denaturing modifier that solubilizes insoluble inclusion bodies of urea and urea-decomposed isocyanates can result in free carbamylation of polypeptide chains, particularly at Alkaline pH when the long-term insulation. SDS, n-hexadecyltrimethylammonium chloride, Sarkosyl, etc., are detergents that can disrupt hydrophobic bonds in proteins and also solubilize some inclusion body proteins. In addition, for cysteine-containing proteins, isolated inclusion bodies typically contain some disulfide bonds formed between the chains and non-reactive disulfide bonds within the chain. Reducing agents such as mercaptoethanol, dithiothreitol (DTT), dithioerythritol and cysteine are also added. For the solubilization of certain inclusion bodies without the disulfide bond of the target protein, the use of reducing agents is sometimes necessary, possibly due to the inclusion body disulfide-solubilizing inclusions.

4,8 M urea dissolved in the inclusion body solution should be how to save?

I put in 4 degrees for half a month, no problem at present

5,8 M Urea dissolved inclusion body solution at room temperature how long can be placed without problems?

BI solution at room temperature for 48 hours, may affect the target protein, because urea can make some amino acids acylation under alkaline conditions, so earlier treatment BI solution is better. What is the specific impact of me is not very clear.